human ifn gamma elispot basic kit alp  (Mabtech Inc)

Journal: bioRxiv

Article Title: Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell Receptors

doi: 10.64898/2026.01.29.702605

Figure Lengend Snippet: Breadth and magnitude of HIV-specific CTL responses in PBMCs, measured by IFN-g ELISPOT to five clade C HLA-B*58:01-restricted consensus epitopes (TW10, IW9, KW11, RY11, and HW9) in the 12 participants, measured during the acute phase of HIV-1 infection. Reported values represent spot-forming units (SFUs) after subtraction of background activity from triplicate negative control wells without peptide stimulation . Each column corresponds to an individual epitope, with response magnitudes depicted by a color scale. Numbers within each box indicate SFUs per 10 6 PBMCs. The intensity scale shown on the right denotes the strength of responses above background. Early and late treated persons are indicated by identifiers shown in red and blue boxes, respectively.

Article Snippet: IFN-gamma ELISPOT assays were performed according to the manufacturer’s instructions with the human IFN-gamma ELISPOT Basic Kit (ALP) (Cat. 3420-2 A, Mabtech).

Techniques: Enzyme-linked Immunospot, Infection, Activity Assay, Negative Control

Journal: Oncoimmunology

Article Title: Isolation of a tumor neoantigen specific CD8+ TCR from a skin biopsy of a vaccination site

doi: 10.1080/2162402X.2025.2457793

Figure Lengend Snippet: Identification of neoantigen-specific T cells in PBMC and VILs. (a). Quantification of IFNγ-secreting cells by ELISpot after challenge with peptides from the vaccine. A post-vaccination sample (left panel, blood 2 in figure 1) and the VILs (right panel) generated from the vaccination site biopsies at day 12 after culture establishment in high IL-2 (as in figure 2A). For the peripheral blood analysis 500,000 PBMC were seeded per well, while for VILs analysis 20,000 effector cells (VILs) were seeded on 100,000 autologous APC. Mean and SEM are shown. (b). IFNγ ELISpot after IVS of the original culture established from the biopsies and divided into bulk (all cells in VILs as in figure 2A) or purified CD8 T cells. Long peptides selected from reactivities found in PBMC and VILs (a) were used for the IVS (as a pool). After IVS cells were tested for reactivity against individual peptides, as indicated. For positive control, anti-CD3 coated wells, and for negative control, human 10 µg/ml human IgG was used. Experimental replicates for tests and controls are shown. (c). Bulk culture after IVS with positive reactivity to peptides (b) were further explored by exploration of immune activation markers by FACS to re-confirm ELISpot results and discriminate between reactive CD4 + and CD8 + T cell populations. Examples for reactive CD4 + (upper and lower panel) and CD8 + (middle panel) T cell populations are shown.

Article Snippet: INF-gamma detection was performed using Human IFN-γ ELISpot basic kit (ALP) (Mabtech) following manufacturer's protocol.

Techniques: Enzyme-linked Immunospot, Generated, Purification, Positive Control, Negative Control, Activation Assay